By G.P. Ellis, G.B. West (Eds.)
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Extra info for Progress in Medicinal Chemistry, Vol. 15
The use of methionine (I3C-methyl) and sodium [ 1 3C]propionate showed that geldanamycin followed the same biosynthetic pathway as rifamycin and streptovaricin D [2 181. Lasalocid A , an antibiotic produced by Streptomyces lasaliensis, is unique in that it is a polyether antibiotic and that it contains 3 C-ethyl groups. Studies on the incorporation of sodium [1-"Clacetate, sodium [l-'3C]propionate, and sodium [ l-13C]butyrate, and examination of the labelled antibiotic with 13CNMR, confirmed that the lasalocid A skeleton was derived from 5 acetate, 4 propionate, and 3 butyrate residues.
Finally, organic samples requiring analysis of 13C isotopic content, may be combusted in a stream of oxygen to provide the carbon dioxide for isotopic analysis. Several combustion systems have been used that differ only in minor components designed to remove contaminants such as sulphur or the halogens [97,98]. In 'C isotopic analysis where the greatest accuracy is required, correction must be made for the contribution of 1zC'60170 to the mass 45 peak in reference and sample gases . Despite the increasing use of the heavy isotope of nitrogen not only in clinical investigations but also in animal and agricultural studies, analytical techniques have not developed to any marked degree over the last few decades.
Cerebral oxygen metabolism has been studied in vivo in different behavioural situations using gas mixtures of oxygen-18 with natural nitrogen . A special air-tight operant conditioning (Skinner) box has been described that enables oxygen metabolism of rats t o be measured using oxygen-18 . CHZCOZH HO ~CH2CH(NHR)C'802Et H (25) (26) The rate of bicarbonate exchange across red cell membranes using rat erythrocytes has been measured with the aid of '80-labelled bicarbonate . When the mechanism of the hydrolysis of sucrose by a sucrase-isomaltase D.