By P. Wyss, Y. Tadir, B.J. Tromberg, U. Haller
The photodynamic approach comprises the management of a photosensitizer by means of program of laser gentle. whilst mild interacts intracellularly with the photosensitizer, the photon power absorbed by means of the sensitizing molecule regularly generates photooxidation for tissue destruction or fluorescence for diagnostics. Photomedical innovations offer a minimally invasive, ambulatory approach in gynecology, now not wanting anesthetics. A historical past of photomedicine explains the most components and mechanisms of photomedical suggestions. one of many major chapters is devoted to the biochemical and biophysical facets of photomedicine resembling characterization of photosensitizers, their interplay with gentle, their destruction, the optical dosimetry and optical homes of tissues. The scientific a part of this publication summarizes the cutting-edge job in all significant gynecologic purposes, from cervix to breast, and gives the reader with an figuring out of photodynamic remedy and easy mechanisms. Photon-assisted replica, e.g. laser-assisted fertilization, embryo hatching and prenatal analysis, makes up the ultimate chapters. Belonging in each health center or college library, this ebook underscores why photomedicine appeals to this kind of huge spectrum of scholars, uncomplicated scientists, clinicians, and contributors of the company global.
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Extra info for Photomedicine in Gynecology & Reproduction
Aggregation of monomers and of sandwich-type dimers can occur for pure, symmetrical regioisomers where building blocks can be repeated with minimal steric perturbations. For AlPcS4, the decrease in the ratio A644/A672 with concentration implies formation of oligomers. Moreover, the bathochromic shift of the Soret band with increasing concentration suggests branched-chain formation . The commercial preparations AlPcS4mix and AlPcS3mix do not form dimers and aggregates but, as expected, AlPcS2mix molecules do dimerize in ‘sandwich’-type structures.
Necrotic processes are probably the consequence, whereas hydrophilic photosensitizers act in a different way. This hypothesis was further supported by the different Ca2+ dynamics which was induced during PDT. In the case of AlPcS4, synchronous to the significant fluorescence increase in the photosensitizer, we observed nonlinear Ca2+ dynamics inside the cell nucleus during the light-induced process which was correlated with apoptotic processes (in preparation). The kinetics of this process, which was analyzed with LSM, is demonstrated in figure 4.
Kimel/Orenstein/Lavie 36 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 Diwu Z, Lown JW: Photosensitization with anticancer agents. 17. EPR studies of photodynamic action of hypericin: Formation of semiquinone radical and activated oxygen species on illumination. Free Radical Biol Med 1993;14:209–215. Meruelo D, Degar S, Amari N, Mazur Y, Lavie D, Levin B, Lavie G: Mode of action of hypericin as an antiretroviral agent and other relevant findings; in Chu CK, Cutler H (eds): Natural Products as Antiviral Agents.