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By H. S. Luker and A. J. Luker (Auth.)

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Extra resources for Laboratory Exercises in Zoology

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Plot a graph of time for hydrolysis against pH. PART C Microscopical Preparations 1. NOTES ON THE PROCESS OF MAKING PERMANENT STAINED PREPARATIONS A permanent preparation should remain suitable for microscopic examination for many years. The different stages involved in making such a preparation are outlined in sequence below. 1. FIXATION The object to be stained is immersed in a fluid known as a fixative. A fixative is satisfactory if it fulfils the following requirements :— (a) It preserves a tissue with the minimum of distortion.

Perform a control test using water in place of the milk sample and note any difference in results. The results of these two tests on milk samples should show that milk contains both reducing sugars and proteins. Leave your test tubes in the rack for comparison with later results. After 2 h or longer—Remove samples of the liquid in the gas jar outside the Visking tubing and perform the following tests. (1) Benedict's test. 8, page 20). (2) Cole's modification of Millon's test. (3) To 3 cm 3 of the solution add 3 cm 3 of 10% aqueous trichloroacetic acid (Caution—prevent contact of this reagent with the skin).

Pour a drop of this liquid on to a piece of filter paper and leave to dry. Note the translucent patch left after the ether has evaporated. 18. REACTION WITH SUDAN IV Reagent—Sudan IV 5 g in 95 cm3 70% alcohol 28 LABORATORY EXERCISES IN ZOOLOGY Procedure When a few drops of olive oil are shaken up in about 2 cm 3 water to form a temporary emulsion and a few drops of Sudan IV are added it will be seen that if the liquid is left to stand the oil which separates out on the top will be stained red by the Sudan IV.

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