By Anurag Rathore, Andras Guttman
Electrokinetic Phenomena emphasizes the influence of equipment comparable to capillary quarter electrophoresis, capillary electrochromatography, and capillary gel electrophoresis at the research of biomolecules. This reference finds the electrokinetic phenomena that underlie high-performance electro-based analytical instruments and vividly depicts how electrodriven analytical instruments revolutionize and expedite chemical, pharmaceutical, and biotechnological research. An authoritative assessment, the e-book offers powerful pathways for large-scale biomedical purposes and describes how microfabricated and automatic units improve and speed up the research of biologically vital molecules
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Additional info for Electrokinetic Phenomena: Principles and Applications in Analytical Chemistry and Microchip Technology
The use of metal ion-supplemented buffers to enhance the resolution of peptides in capillary zone electrophoresis. Electrophoresis 1990; 11:765. Lloyd DK, Waetzig H. Sodium dodecyl sulfate is an effective between-run rinse for capillary electrophoresis of samples in biological matrixes. J Chromatogr B: Biomed Appl 1995; 663:400. Lurie IS, Bethea MJ, McKibben TD, Hays P, Pellegrini P, Sahai R, Garcia A, Weinberger R. Use of dynamically coated capillaries for the routine analysis of methamphetamine, amphetamine, MDA, MDMA, MDEA and cocaine using capillary electrophoresis.
Capillary. Once a good separation is obtained, try shortening the capillary length. If broad peaks are noted during these scouting runs, the solute may be adhering to the capillary wall. This is commonplace when polycations are being separated. Before any further optimization can be performed, this problem must be dealt with. For low-pH separations, a coated capillary can be tried. Alternatively, the charge on the capillary wall can be reversed with a cationic surfactant  or a polycation .
26. 27. 28. 29. 30. 31. 32. 33. Copyright © 2004 Marcel Dekker, Inc. Methods Development for Capillary Electrophoresis 34. 35. 36. 37. 38. 39. 40. 41. 42. 43. 44. 45. 46. 47. 48. 49. 50. 51. 41 Mosher RA. The use of metal ion-supplemented buffers to enhance the resolution of peptides in capillary zone electrophoresis. Electrophoresis 1990; 11:765. Lloyd DK, Waetzig H. Sodium dodecyl sulfate is an effective between-run rinse for capillary electrophoresis of samples in biological matrixes. J Chromatogr B: Biomed Appl 1995; 663:400.